The UK CARES analytical core is equipped with a range of GC and LC coupled mass spectrometer systems including multistage and high resolution instruments. These instruments are used for quantitation of small molecules of broad relevance to environmental disease researchers.
The laboratory contains two Agilent GC MS systems with quadrupole and triple quadrupole mass analyzers as well as electron capture and flame ionization detectors. It contains three ABSciex triple quadrupole linear ion tram mass spectrometer systems with Shimadzu HPLC and UPLC systems as well as an ABSciex quadrupole time of flight mass spectrometer system with HPLC and microflow chromatography systems and a Thermo Q-Exactive quadripole orbitrap mass spectrometer with a dionex UPLC system.
The laboratory is also well equipped for sample preparation from biological and environemental matrices with automated systems for solid phase and accelerated solvent extraction as well as solvent evaporation including several Caliper Turbovap evaporators and a large volume genevac solvent evaporator.
The laboratory can conduct measurements of many different classes of pollutants in biological or environmental matrices including serum and blood plasma, tissues, water and soil. Particular areas of emphasis include polychlorinated biphenyls, dioxins, polychlorinated benzodifurans, per- and polyfluoroalkyl substances, and bisphenols. Published methods for other established and emerging persistent organic pollutants and biomarkers of exposure to these pollutants can be set up and validated for use in preclinical or clinical studies as needed.
Director: Andrew J. Morris, PhD
Co-Leader: Jason Unrine, PhD
Staff: M. Abdul Mottaleb, PhD, Sony Soman, PhD, Courtney Hammill, MS
Location: B074 BBSRB 741 South Limestone, Lexington KY 40536
Phone: 859 323 3749
- Petriello MC, Hoffman JB, Sunkara M, Wahlang B, Perkins JT, Morris AJ, Hennig B. Dioxin-like pollutants increase hepatic flavin containing monooxygenase (FMO3) expression to promote synthesis of the pro-atherogenic nutrient biomarker trimethylamine N-oxide from dietary precursors. The Journal of nutritional biochemistry. 2016;33:145-53. Epub 2016/05/09. doi: 10.1016/j.jnutbio.2016.03.016. PMC4893916
- Murphy MO, Petriello MC, Han SG, Sunkara M, Morris AJ, Esser K, Hennig B. Exercise protects against PCB-induced inflammation and associated cardiovascular risk factors. Environmental science and pollution research international. 2016;23(3):2201-11. Epub 2015/01/15. doi: 10.1007/s11356-014-4062-6. PMC4503535
- Baker NA, Shoemaker R, English V, Larian N, Sunkara M, Morris AJ, Walker M, Yiannikouris F, Cassis LA. Effects of Adipocyte Aryl Hydrocarbon Receptor Deficiency on PCB-Induced Disruption of Glucose Homeostasis in Lean and Obese Mice. Environmental health perspectives. 2015;123(10):944-50. Epub 2015/03/04. doi: 10.1289/ehp.1408594. PMC4590748
- Newsome BJ, Petriello MC, Han SG, Murphy MO, Eske KE, Sunkara M, Morris AJ, Hennig B. Green tea diet decreases PCB 126-induced oxidative stress in mice by up-regulating antioxidant enzymes. The Journal of nutritional biochemistry. 2014;25(2):126-35. Epub 2014/01/01. doi: 10.1016/j.jnutbio.2013.10.003. PMC3946959
- Sui Y, Park SH, Helsley RN, Sunkara M, Gonzalez FJ, Morris AJ, Zhou C. Bisphenol A increases atherosclerosis in pregnane X receptor-humanized ApoE deficient mice. Journal of the American Heart Association. 2014;3(2):e000492. Epub 2014/04/24. doi: 10.1161/jaha.113.000492. PMC4187496
- Baker NA, Karounos M, English V, Fang J, Wei Y, Stromberg A, Sunkara M, Morris AJ, Swanson HI, Cassis LA. Coplanar polychlorinated biphenyls impair glucose homeostasis in lean C57BL/6 mice and mitigate beneficial effects of weight loss on glucose homeostasis in obese mice. Environmental health perspectives. 2013;121(1):105-10. Epub 2012/10/27. doi: 10.1289/ehp.1205421. PMC3553436
- Baker NA, English V, Sunkara M, Morris AJ, Pearson KJ, Cassis LA. Resveratrol protects against polychlorinated biphenyl-mediated impairment of glucose homeostasis in adipocytes. The Journal of nutritional biochemistry. 2013;24(12):2168-74. Epub 2013/11/16. doi: 10.1016/j.jnutbio.2013.08.009. PMC4066417
- Majkova Z, Layne J, Sunkara M, Morris AJ, Toborek M, Hennig B. Omega-3 fatty acid oxidation products prevent vascular endothelial cell activation by coplanar polychlorinated biphenyls. Toxicology and applied pharmacology. 2011;251(1):41-9. Epub 2010/12/07. doi: 10.1016/j.taap.2010.11.013. PMC3026064